Protein kinase C activation upregulates human L-type amino acid transporter 2 function

Abstract L-type amino acid transporter 2 (LAT2) is a Na + -independent neutral transporter, whose function regulation system remains unclarified. Since protein kinase C (PKC) known to regulate the functions of various transporters, we investigated whether human LAT2 (hLAT2) regulated by PKC. In mouse proximal tubule S2 cells, hLAT2 transport activity was upregulated PKC activation. However, found that mRNA and expression not affected activation upregulation independent three potential consensus sites in sequence. Moreover, Vmax value for hLAT2-mediated alanine transport, which accompanied induction membrane insertion. conclusion, showed activation, related either de novo synthesis, phosphorylation or insertion hLAT2.